Journal: Journal of virology

Article Title: Evidence for a stable interaction of gp41 with Pr55(Gag) in immature human immunodeficiency virus type 1 particles.

doi: 10.1128/jvi.74.20.9381-9387.2000

Figure Lengend Snippet: FIG. 5. Association of HIV-1 Env proteins on immature HIV-1 cores inde- pendently of the presence of lipid rafts. Immature HIV-1 cores were isolated from a PLAP-encoding virus by incubation for 1 h in detergent at either 4 or 37°C, followed by sedimentation centrifugation. Fractions were examined for the presence of PLAP and HIV-1 Env proteins. (A and B) Levels of PLAP were determined by incubation of gradient fractions with an AP substrate and quan- titated on a luminometer as relative units (RU) of luminescence. A CA-specific ELISA was used to determine the amount of virus in each gradient fraction. (C) Immunoblot analysis of the peak Pr55Gag-containing gradient fractions from immature cores treated with detergent at either 4 or 37°C (lanes 1 and 4, respectively) was performed as described in the legend to Fig. 2. Threefold serial dilutions of the peak Pr55Gag-containing fractions were also analyzed (lanes 2 and 3 and lanes 5 and 6).

Article Snippet: Immunoblot analysis was performed on the pelleted gradient fractions as mentioned above using rabbit antiserum against human PLAP (obtained from Fitzgerald International Industries, Inc.).

Techniques: Isolation, Virus, Incubation, Sedimentation, Centrifugation, Enzyme-linked Immunosorbent Assay, Western Blot